Bacteriology

Bacteriology

The Bacteriology laboratory analyzes samples taken from patients hospitalized or consulting at the Caen Normandy University Hospital, in search of pathogenic bacteria.

Team

Service composition

2 university professors · hospital practitioners
1 university lecturer · hospital practitioner
3 hospital practitioners
1 hospital-university assistant
1 healthcare manager
1 senior health executive

Department of Infectious Agents

University Professor · Hospital Practitioner
Professor Simon Le Hello
Head of the Biology Laboratory andITBS
University Professor · Hospital Practitioner
Professor Olivier Join-Lambert
Biologist
University Lecturer · Hospital Practitioner
Dr. Christophe Isnard
Biologist
Hospital Practitioner
Dr. Marguerite Fines-Guyon
Biologist
Hospital Practitioner
Dr. Claire Daurel
Biologist
Hospital Practitioner
Dr. Damasie Malandain
Biologist
University Hospital Assistant
Dr. François Gravey
Biologist
healthcare manager
Natacha Hurel

Professor Simon Le Hello

Head of department

Manuela Leroux

Senior healthcare executive

Our services

Activities

The Microbiology sector encompasses diagnostic activities in Bacteriology, Mycology, and Parasitology at the Côte de Nacre site. It is organized into 7 sub-sectors:

Syndromic PCR
Bacterial culture
Identification and antibiogram
Mycobacteria (P3 laboratory)
Bacterial, fungal and parasitic serology
Molecular Biology
Genomic epidemiology

Bacteriological diagnosis and therapeutic advice

Performing bacteriological analyses: direct examinations, cultures, identification by MALDI-TOF mass spectrometry, antigenic, serological and molecular methods (PCR , bacterial "pan")
Classic and rapid diagnosis (by molecular biology) of tuberculosis; diagnosis of atypical mycobacterial infections, mycobacterial antibiograms
Determination of antibiotic susceptibility (antibiogram), including the reference method by microdilution in liquid medium (Determination of Minimum Inhibitory Concentrations) (VIZION System)
role of alert and therapeutic advice in case of diagnosis of severe infections (bacteremia, endocarditis…).

Prevention of infectious risk:

Identification of multi-resistant (MRS) and emerging highly resistant (HR) bacteria.
Infection Prevention and Control Service

24/7 diagnosis of bacterial infections (continuous care),
Performing antibiotic susceptibility tests: disk diffusion antibiogram and liquid medium, minimum inhibitory concentration (MIC)
First-line therapeutic advice (antibiotics, including antifungals and antiparasitics)
Participation in the Anti-Infective Drugs Commission (CAI) for the appropriate prescription of antibiotics, antifungals and antiparasitics
Involvement in the prevention and control of healthcare-associated infections through the detection, identification and molecular typing of multidrug-resistant (MDR) and highly resistant (HR) bacteria in collaboration with the Infection Prevention and Control service

Prevention and control of nosocomial infections: identification and typing of multi-resistant bacteria in collaboration with the Infection Prevention and Control.

Member of FeNOMIH (Normandy University Hospital Federation of Microbiology, Infectious Diseases and Hospital Hygiene)
Laboratory associated with the Reference Centre for Complex Osteoarticular Infections.
Regional Genomic Epidemiology Unit (CREM) : centralization in the laboratory of highly resistant strains, or any strain responsible for an epidemic in the Normandy region, for molecular analysis and understanding of the mechanisms of strain circulation
Reference laboratory for prenatal diagnosis of toxoplasmosis (ministerial approval).
Territorial Institute of Health Biology

Areas of expertise

Diagnosis and antibiotic therapy of osteoarticular infections, infections of intracardiac material and infective endocarditis, infections in subjects with cystic fibrosis, infections with tuberculous and atypical mycobacteria
Antibiotic resistance (phenotypic and molecular diagnosis)
Molecular analysis

Microbiological control of health products

Microbiological control of health products (grafts, breast milk, biotherapy).
A forensic medical unit was requisitioned for the analysis of samples in a legal context.

Centers & platforms

	Rare Disease Reference Center Complex Osteoarticular Infection	Center page
	Platform Infectious platform	Center page
	Structure Department of Infectious Agents	Center page

Teaching and research

Research

Due to its expertise and research on pathogens and infectious diseases, the Microbial Adaptation Research Group ( GRAM 2.0) has been awarded INSERM accreditation, leading to the creation of the Joint Research Unit UMR 1311 DYNAMICURE for Microbial Dynamics Associated with Urinary and Respiratory Infections. Established on January 1 ^, 2022, the UMR is being restructured by integrating fundamental bacteriologists from the University of Caen Normandy (UCN) and staff from Labéo (an animal health research center), while focusing its scientific work on urinary and respiratory infections.

It brings together teacher-researchers from the laboratories of:

Virology (Professor A. VABRET, Caen Normandy University Hospital, Professor JC PLANTIER, Rouen Normandy University Hospital)
Bacteriology (Prof. M. PESTEL-CARON, Rouen Normandy University Hospital, Prof. O. JOIN LAMBERT, Caen Normandy University Hospital),

services from :

Infectious and Tropical Diseases (Prof. R. VERDON, Caen Normandy University Hospital, Prof. F. CARON, Rouen Normandy University Hospital),
Pediatrics (Professor J. BROUARD, Caen Normandy University Hospital)
Intensive Care Unit (Professor B. MISSET, Rouen Normandy University Hospital)

and the Department for Clinical Research and Innovation (Pr. JJ PARIENTI, CHU Caen Normandie).

The main research areas of the Microbiology laboratory are:

the study of virulence and antibiotic resistance mechanisms of multidrug-resistant bacteria responsible for epidemics and nosocomial infections,
The molecular pathophysiology and management of complicated skin infections (including Verneuil's disease or hidradenitis suppurativa), using the opportunistic pathogen Staphylococcus lugdunensis

To answer these questions, the department is currently developing a next-generation sequencing (NGS) approach in close collaboration with the Virology andHospital Hygiene.

teaching

The practitioners of the service participate in the theoretical training of students from the Faculty of Medicine (UE Infectious Agents) and the Faculty of Pharmacy, interns of the DES in Medical Biology, students in Midwifery, operating room nurses, students in BTS, in Sciences (Bachelor's, Master's, Doctorates, university diplomas…).

Practical training in the laboratory

Due to its hospital-university activities, the department is accredited for the practical training of interns enrolled in the DES (Diploma of Specialized Studies) in Medical Biology

He is also involved in the training of technical staff, interns outside the field, trainees in BTS, licentiates, masters, and various health professionals as part of continuing education.