Sample catalog · Innovabio - Caen Normandy University Hospital

Catalog of biological collections

The InnovaBIO Biological Resource Center offers you its online catalog which allows you to have access to the biological resources in the bank and to the techniques offered.

Professor Denis Vivien
Head of CRB InnovaBIO

Catalog of biological collections of the CRB on data.gouv.fr

Neurology & Infectious Diseases

BIO STROKE

Acute ischemic or hemorrhagic neurovascular pathology

Sample types:

plasma citrate

Responsible: Richard Macrez

Covid -19 CRB

covid-19

Sample types:

bronchial aspiration
tracheal suction
IBA (bronchoalveolar fluid)
right lba
left lba
peritoneal dialysis fluid ( PDF )
pleural fluid
pdp (protected distal sampling)
plasma citrate
EDTA plasma
fluorinated plasma
heparin plasma
heparin gel plasma
dry serum with gel
dry serum without gel

Manager: Denis Vivien

MAESTRO

Transient cerebral ischemia

Sample types:

plasma citrate
EDTA plasma

Manager: Emmanuel Touzé

Techniques used

Serum

Type of tubes	Centrifugation	Volume of serum obtained	Sampling	Conservation
	2000 g 10 minutes	≅ 1 – 1.5 ml	Recommended volumes: 200-500 μL/aliquots	-80°C

Plasma

Type of tubes	Centrifugation	Volume of plasma obtained	Sampling	Conservation
	2300 g 11 minutes (room temperature) Double centrifugation for citrate tubes = platelet-free plasma Possibility of collecting the buffy coat at the interface of the plasma and the cell pellet.	≅ 1.5 – 2 ml	Recommended volumes: 200-500 μL/aliquots	-80°C

Isolation of PBMCs

Dilution in PBS	Centrifugation	PBS rinses	Cell counting and viability	Conservation
Yes (1:2)	400 g – 30 mins without brake, at room temperature	500 g – 10 min	Luna II with trypan blue /erythrosine	Container for progressive freezing at -80°C then storage in liquid nitrogen (-196°C)
Yes (1:2)	1200 g – 10 min with brake, at room temperature	300 g – 8 min	Luna II with trypan blue /erythrosine	Container for progressive freezing at -80°C then storage in liquid nitrogen (-196°C)
Yes (1:2 to 14)	1000 g – 800 g 10 – 15 min without brake, at room temperature	250 g – 10 min	Luna II with trypan blue /erythrosine	Container for progressive freezing at -80°C then storage in liquid nitrogen (-196°C)
No	1500 g – 1800 g 15 – 20 min without brake, at room temperature	300 g 10-15 min	Luna II with trypan blue /erythrosine	Container for progressive freezing at -80°C then storage in liquid nitrogen (-196°C)

Number of cells or cryotubes varies depending on the initial blood volume and the technique used (Contact us)

Biological Resource Center InnovaBIO