Sample catalogue · Innovabio - Caen Normandy University Hospital

Catalogue of biological collections

The InnovaBIO Biological Resource Centre offers you its online catalogue which allows you to access biological resources in the bank and the techniques offered.

Professor Denis Vivien,
Head of the Biological Resource Center InnovaBIO

Catalogue of biological collections of the CRBs on data.gouv.fr

Neurology & Infectious Diseases

BIO STROKE

Acute ischemic or hemorrhagic neurovascular pathology

Sample types:

plasma citrate

Manager: Richard Macrez

Covid-19 CRB

Covid-19

Sample types:

bronchial aspiration
tracheal aspiration
BAL (bronchoalveolar fluid)
lba right
left
peritoneal dialysis fluid (PDF)
pleural fluid
pdp (protected distal sampling)
plasma citrate
EDTA plasma
fluoride plasma
plasma heparin
heparin gel plasma
dry serum with gel
dry serum without gel

Manager: Denis Vivien

MAESTRO

Transient cerebral ischemia

Sample types:

plasma citrate
EDTA plasma

Manager: Emmanuel Touzé

Techniques used

Serum

Tube type	Centrifugation	Serum volume obtained	Sampling	Conservation
	2000 g 10 minutes	≅ 1 – 1.5 ml	Recommended volumes: 200-500 μL/aliquots	-80°C

Plasma

Tube type	Centrifugation	Plasma volume obtained	Sampling	Conservation
	2300 g 11 minutes (room temperature) Double centrifugation for citrate tubes = free plasma in platelets. Possibility of collecting the buffy coat at the interface of the plasma and the cell pellet.	≅ 1.5 – 2 ml	Recommended volumes: 200-500 μL/aliquots	-80°C

Isolation of PBMCs

Dilution in PBS	Centrifugation	PBS rinses	Cell counting and viability	Conservation
Yes (1:2)	400 g – 30 min without braking, at room temperature	500 g – 10 min	Luna II with trypan blue/erythrosine	freezing container at -80°C followed by storage in liquid nitrogen (-196°C)
Yes (1:2)	1200 g – 10 min with brake, at room temperature	300 g – 8 min	Luna II with trypan blue/erythrosine	freezing container at -80°C followed by storage in liquid nitrogen (-196°C)
Yes (1:2 to 14)	1000 g – 800 g 10 – 15 min without braking, at room temperature	250 g – 10 min	Luna II with trypan blue/erythrosine	freezing container at -80°C followed by storage in liquid nitrogen (-196°C)
No	1500 g – 1800 g 15 – 20 min without braking, at room temperature	300 g 10-15 min	Luna II with trypan blue/erythrosine	freezing container at -80°C followed by storage in liquid nitrogen (-196°C)

The number of cells or cryotubes varies depending on the initial blood volume and the technique used (Please contact us)

Biological Resource Center · InnovaBIO