Skip to main content
Biothu00e8que Biothu00e8que
CHU Caen Normandy

Catalog of biological collections

The InnovaBIO Biological Resource Center offers you its  online catalog  which allows you to have access to the biological resources in the bank and to the techniques offered.

The head of the BRC InnovaBIO , Professor Denis Vivien

Neurology & infectious

ORGANIC STROKE

Acute ischemic or hemorrhagic neurovascular pathology
Types of samples:
  • plasma citrate
Responsible: Richard Macrez

Covid -19 CRB

covid-19
Types of samples:
  • bronchial aspiration
  • tracheal suction
  • lba (broncho alveolar fluid)
  • straight lba
  • left lba
  • peritoneal dialysis fluid ( ldp )
  • pleural fluid
  • pdp (protected distal sampling)
  • plasma citrate
  • EDTA plasma
  • fluorinated plasma
  • plasma heparin
  • plasma heparin gel
  • dry serum with gel
  • gel-free dry serum
Responsible: Denis Vivien

MAESTRO

Transient cerebral ischemia
Types of samples:
  • plasma citrate
  • EDTA plasma

Techniques used

Serum

Tube typecentrifugationVolume of serum obtainedSamplingConservation

2000g
10 mins

≅ 1 – 1.5ml

Recommended volumes:
200-500 μL/aliquots

-80°C

Plasma

Tube typecentrifugationVolume of plasma obtainedSamplingConservation

2300 g
11 minutes (room temperature)

Double centrifugation for citrate tubes = free plasma in platelet
Possibility of taking the buffy coat at the interface of the plasma and the cell pellet.

≅ 1.5 – 2ml

Recommended volumes:
200-500 μL/aliquots

-80°C

Isolation of PBMCs

Tube typeDilution in PBScentrifugationPBS rinsesCell count and viabilityConservation

Yes
(1:2)

400 g – 30 min
without brake, in ambient

500 g – 10 mins

Luna II with
trypan blue/erythrosine

freezing container
at -80°C then
storage in
liquid nitrogen (-196°C)

Yes
(1:2)

1200 g – 10 min
with brake, ambient

300 g – 8 mins

Luna II with
trypan blue/erythrosine

freezing container
at -80°C then
storage in
liquid nitrogen (-196°C)

Yes
(1:2 to 14)

1000 g – 800 g
10 – 15 min
without brake, ambient

250 g – 10 mins

Luna II with
trypan blue/erythrosine

freezing container
at -80°C then
storage in
liquid nitrogen (-196°C)

No

1500 g – 1800 g
1520 min
without brake, ambient

300 g
10-15 mins

Luna II with
trypan blue/erythrosine

freezing container
at -80°C then
storage in
liquid nitrogen (-196°C)

 Number of cells or variable cryotubes depending on the initial blood volume and the technique used (contact us)